产品说明书
Jervine
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同义名 : | 蒜藜芦碱 ;11-Ketocyclopamine;NSC 7520;NSC 23898 |
| CAS号 : | 469-59-0 | |
| 货号 : | A160362 | |
| 分子式 : | C27H39NO3 | |
| 纯度 : | 98% | |
| 分子量 : | 425.603 | |
| MDL号 : | MFCD01684066 | |
| 存储条件： |
粉末 Sealed in dry,Store in freezer, under -20°C 液体 -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
DMSO: 1 mg/mL(2.35 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO |
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| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | Hedgehog (Hh) is one of few of signaling pathways that is frequently used during development for intercellular communication. Hh is important for the organ oogenesis of almost all organs in mammals, as well as in regeneration and homeostasis. Further, Hh signaling is disrupted in diverse types of cancer. Jervine, also called 11-Ketocyclopamine, is hedgehog signaling inhibitor with IC50 of 500 - 700 nM. Tongues cultured with 5 μM cyclopamine or jervine had an increased density of fungiform papillae on the anterior tongue, and numerous fungiform papillae on the posterior tongue near the circumvallate papilla, compared with 5 μM solanidine-treated group[5]. Oral administration of jervine (50-400 mg/kg) exerted 50.4-73.5% anti-inflammatory effects in carrageenan induced paw edema and inflammation biomarkers such as TNF-α, IL-1β and MPO that increased by carrageenan injection were suppressed by the administrations of all doses of jervine[6]. It was found that jervine significantly increased external granule layer (proliferative layer producing over 90% of cerebellar neurons) apoptosis at 50 mg/kg and above in a dose dependent manner[7]. | ||
| 细胞研究 | |||||
|---|---|---|---|---|---|
| 细胞系 | 浓度 | 检测类型 | 检测时间 | 活动说明 | 数据源 |
| s12 cells | Function assay | Inhibition of lipid-modified (octylated) form of SHH expressed in mouse s12 cells by luciferase reporter gene assay, IC50=0.5 μM | 19309080 | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
2.35mL 0.47mL 0.23mL |
11.75mL 2.35mL 1.17mL |
23.50mL 4.70mL 2.35mL |
| 参考文献 |
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