2',3'-cGAMP sodium

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Chemical Structure| 2734858-36-5 同义名 : 2'-3'-cyclic GMP-AMP sodium
CAS号 : 2734858-36-5
货号 : A1551578
分子式 : C20H22N10Na2O13P2
纯度 : 99%
分子量 : 718.375
MDL号 : MFCD28580126
存储条件：

Pure form Keep in dark place,Inert atmosphere,Store in freezer, under -20°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

H2O: 50 mg/mL(69.6 mM)，配合低频超声助溶
动物实验配方：
生物活性
描述 Stimulator of interferon genes (STING; encoded by TMEM173) is a signaling molecule associated with the endoplasmic reticulum (ER) and is essential for controlling the transcription of numerous host defence genes. [1]. 2',3'-cGAMP is a second messenger that binds and activates the adaptor protein STING with a Kd value of 3.79 nM, which triggers the innate immune response. It is a potent inducer of IFN-β mRNA expression with an EC50 value of 19.4 nM in L929 cells. [2]. Coinjection of 2′3′-cGAMP (1mg/m; intrathoracic injection) with DCV or the related CrPV resulted in a significant decrease in viral RNA accumulation in WT flies. Accordingly, 2′3′-cGAMP improved the survival of DCV-infected WT flies[3]. Thousands of genes were induced by 2’3’-cGAMP, many of which are homologs of genes known to function in mammalian immunity in N. vectensis after 24 h treatment (500 μM in bath of 1/3× sea water). Immune gene induction downstream of 2',3'-cGAMP also occur in embryo with lower dose and shorter treatment (100 μM in bath of 1/3× sea water)[4].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.39mL

0.28mL

0.14mL

6.96mL

1.39mL

0.70mL

13.92mL

2.78mL

1.39mL
参考文献

[1]Barber, G. N. STING: infection, inflammation and cancer. Nat Rev Immunol. 2015; 15(12): 760-770.

[2]Zhang, X., Shi, H., Wu, J., et al. Cyclic GMP-AMP containing mixed phosphodiester linkages is an endogenous high-affinity ligand for STING. Mol. Cell. 2015;51(2), 226-235.

[3]Cai, H., Holleufer, A., Simonsen, B. et al. 2',3'-cGAMP triigers a STING- and NF-κB–dependent broad antiviral response in Drosophila. Science Signalling. 2020;12(660).

[4]Margolis, S. R., Dierzen, P. A. et al. The cyclic dinucleotide 2’3’-cGAMP induces a broad antibacterial and antiviral response in the sea anemone Nematostella vectensis. PNAS. 2021;118(5): e2109022118.