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Isochlorogenic acid B

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Chemical Structure| 14534-61-3 同义名 : 金银花 ;3,4-Dicaffeoylquinic acid;3,4-Di-O-caffeoylquinic acid;3,4-DCQA
CAS号 : 14534-61-3
货号 : A149830
分子式 : C25H24O12
纯度 : 99%+
分子量 : 516.451
MDL号 : MFCD08272439
存储条件：

粉末 Sealed in dry,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 50 mg/mL(96.81 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Isochlorogenic acid B (ICAB), naturally isolated from Laggera alata, and has antioxidative and hepatoprotective properties. ICAB significantly improved the pathological lesions of liver fibrosis. ICAB had a significant protective effect on fibrosis in NASH (non-alcoholic steatohepatitis) by inhibiting oxidative stress via Nrf2 and suppressing multiple profibrogenic factors through miR-122/HIF-1α signalling pathway[3]. Isochlorogenic Acid B (3,4-Dicaffeoylquinic Acid) is a polyphenol with diverse biological activities, it inhibits acetylcholinesterase (ACE) and α-glucosidase in a concentration-dependent manner, shows cytotoxic to NCI-H23 lung adenocarcinoma cells (EC50 3.26 μg/ml), as well as increases tumor necrosis factor-related apoptosis inducing ligand (TRAIL) mRNA, reduces influenza H1N1 hemagglutinin (HA) mRNA and increases survival in a mouse model of influenza A infection. 3,4-Dicaffeoylquinic acid also displays a concentration-dependent α-glucosidase inhibition with EC50 241.80 μg/ml[4]. ITPL contained the following seven compounds: neochlorogenic acid, cryptochlorogenic acid, rutin, kaempferin, isochlorogenic acid B, isochlorogenic acid A, and hesperidin. The pathological observations show that ITPL reduced the oxidative damage of the liver and spleen in mice induced with D-galactose. ITPL had beneficial preventive effects on the oxidative damage caused by D-galactose in mice and was more effective as an antioxidant than vitamin C[5].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.94mL

0.39mL

0.19mL

9.68mL

1.94mL

0.97mL

19.36mL

3.87mL

1.94mL
参考文献

[1]Takemura T, Urushisaki T, et al. 3,4-Dicaffeoylquinic Acid, a Major Constituent of Brazilian Propolis, Increases TRAIL Expression and Extends the Lifetimes of Mice Infected with the Influenza A Virus. Evid Based Complement Alternat Med. 2012;2012:946867.

[2]Choi J, Park JK, et al. In vivo antihepatotoxic effects of Ligularia fischeri var. spiciformis and the identification of the active component, 3,4-dicaffeoylquinic acid. J Med Food. 2005 Fall;8(3):348-52.

[3]Liu X, Huang K, Niu Z, Mei D, Zhang B. Protective effect of isochlorogenic acid B on liver fibrosis in non-alcoholic steatohepatitis of mice. Basic Clin Pharmacol Toxicol. 2019 Feb;124(2):144-153

[4]Ooi KL, Muhammad TS, Tan ML, Sulaiman SF. Cytotoxic, apoptotic and anti-α-glucosidase activities of 3,4-di-O-caffeoyl quinic acid, an antioxidant isolated from the polyphenolic-rich extract of Elephantopus mollis Kunth. J Ethnopharmacol. 2011 Jun 1;135(3):685-95

[5]Yi R, Chen X, Li W, Mu J, Tan F, Zhao X. Preventive effect of insect tea primary leaf (Malus sieboldii (Regal) Rehd.) extract on D-galactose-induced oxidative damage in mice. Food Sci Nutr. 2020 Aug 13;8(9):5160-5171