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Akebia saponin D

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Chemical Structure| 39524-08-8 同义名 : 木通皂甙 D ;Asperosaponin VI;ASA VI;Tauroside St-G0-1;Leiyemudanoside A
CAS号 : 39524-08-8
货号 : A149084
分子式 : C47H76O18
纯度 : 98%
分子量 : 929.096
MDL号 : MFCD25371916
存储条件：

粉末 Inert atmosphere,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 25 mg/mL(26.91 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 100 mg/mL(107.63 mM)，配合低频超声助溶
动物实验配方：
生物活性
描述 Asperosaponin VI (ASA VI), a saponin component from Dipsacus asper wall. ASA VI exhibited a significant induction of proliferation, differentiation and mineralization in MC3T3-E1 and primary osteoblastic cells. ASA VI may induce differentiation by increasing the activity of p38 and ERK1/2[3]. ASA VI significantly attenuated increased LDH (lactate dehydrogenase) and CK (creatine phosphokinase) activities, and increased cell viability in hypoxia treated myocytes in a dose-dependent fashion. ASA VI treatment inhibited apoptosis in hypoxia-induced cardiomyocyte by increasing the Bcl-2/Bax ratio and decreasing active caspase-3 expression, as well as enhancing of p-Akt and p-CREB( cAMP response element-binding protein)[4]. ASA VI promoted the proliferation of OVX rBMSCs and enhanced ALP activity and calcified nodule formation. ASA VI promotes the osteogenic differentiation of OVX rBMSCs (osteogenic differentiation of bone marrow stromal cells) by acting on the phosphatidylinositol-3 kinase/AKT signaling pathway[5].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.08mL

0.22mL

0.11mL

5.38mL

1.08mL

0.54mL

10.76mL

2.15mL

1.08mL
参考文献

[1]Song L, Wang S, et al. Chromatographic fingerprint combined with content of asperosaponin VI and antioxidant activity for quality evaluation of wine-fried Dipsaci Radix. Nat Prod Commun. 2014 Jun;9(6):773-8.

[2]Zhang R, Zhu H, et al. Determination of asperosaponin VI and its active metabolite hederagenin in rat tissues by LC-MS/MS: application to a tissue distribution study. J Chromatogr B Analyt Technol Biomed Life Sci. 2014 May 15;959:22-6.

[3]Niu Y, Li Y, Huang H, et al. Asperosaponin VI, a saponin component from Dipsacus asper wall, induces osteoblast differentiation through bone morphogenetic protein-2/p38 and extracellular signal-regulated kinase 1/2 pathway. Phytother Res. 2011;25(11):1700-1706

[4]Li C, Tian J, Li G, et al. Asperosaponin VI protects cardiac myocytes from hypoxia-induced apoptosis via activation of the PI3K/Akt and CREB pathways. Eur J Pharmacol. 2010;649(1-3):100-107

[5]Ke K, Li Q, Yang X, et al. Asperosaponin VI promotes bone marrow stromal cell osteogenic differentiation through the PI3K/AKT signaling pathway in an osteoporosis model. Sci Rep. 2016;6:35233. Published 2016 Oct 19