Trimethyloctadecylammonium bromide
产品说明书
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同义名 : | OctMAB;Octadecyltrimethylammonium bromide;C18TAB |
| CAS号 : | 1120-02-1 | |
| 货号 : | A147673 | |
| 分子式 : | C21H46BrN | |
| 纯度 : | 97% | |
| 分子量 : | 392.501 | |
| MDL号 : | MFCD00043171 | |
| 存储条件： |
Pure form Inert atmosphere,Room Temperature In solvent -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
DMSO: 9 mg/mL(22.93 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO |
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| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | The endocytic protein dynamin II (dynII) is a 100-kDa GTPase enzyme best known for its participation in cell cycle progression and role in centrosome cohesion and cytokinesis. Octadecyltrimethyl ammonium bromide (OctMAB) is a novel inhibitor of dynII GTPase activity with IC50 value of 4.35 ± 2.42 μM. It also inhibits dynI GTPase activity with IC50 value of 0.92 ± 0.13 μM. HeLa, H460, and SW480 cancer cells were treated with various concentrations of OctMAB for 7 d. Antiproliferative effect of OctMAB in these cells with a 50% reduction in colony formation was confiemed at concentration of ∼0.5 μM indicating OctMAB inhibited cell proliferation and reduce viability in a range of cancer cells. Asynchronously growing HeLa cells were treated for 48 h with OctMAB. Consistent with its effect on cell viability, it increased the sub-G1 (<2N DNA content) population, indicating apoptosis. G1 and S phase populations decreased, whereas the population of cells containing 4N DNA content (G2-M) increased. Therefore, OctMAB disrupted cell cycle progression and caused cytokinesis failure [1]. | ||
| 作用机制 | OctMAB inhibits dynamin GTPase activity by disrupting the PH (pleckstrin homology) domain-phospholipid interaction [1]. | ||
| 实验方案 | |||
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| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
2.55mL 0.51mL 0.25mL |
12.74mL 2.55mL 1.27mL |
25.48mL 5.10mL 2.55mL |
| 参考文献 |
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