产品说明书
Colchicine
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同义名 : | 秋水仙素 |
| CAS号 : | 64-86-8 | |
| 货号 : | A145127 | |
| 分子式 : | C22H25NO6 | |
| 纯度 : | 98% HPLC(contains ca.5% EA and 1%ACE and 1%DMSO) | |
| 分子量 : | 399.437 | |
| MDL号 : | MFCD00078484 | |
| 存储条件： |
粉末 Keep in dark place,Inert atmosphere,2-8°C 液体 -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
DMSO: 50 mg/mL(125.18 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO H2O: 30 mg/mL(75.11 mM) |
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| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | Colchicine is a tubulin inhibitor and a microtubule disrupting agent. Colchicine inhibits microtubule polymerization with an IC50 of 3 nM[3]. Exposure to 1 mM colchicine, a microtubule disrupting agent, triggered apoptosis in rat cerebellar granule cells (CGC). Colchicine treatment also causes alterations in Ca2+ responses to chemical depolarization and a moderate, but progressive, increase in the resting intracellular Ca2+ concentration[4]. Colchicine appears to be efficacious and well tolerated for recurrent pericarditis/postpericardiotomy syndrome and recurrence of postprocedural atrial fibrillation[5]. Colchicine treatment inhibited indomethacin-induced small intestinal injury by 86% (1 mg/kg) and 94% (3 mg/kg) as indicated by the lesion index 24 h after indomethacin administration. Colchicine inhibited the protein expression of cleaved caspase-1 and mature IL-1β, without affecting the mRNA expression of NLRP3 and IL-1β[6]. Short-term treatment with colchicine (0.1 mg/kg/day, p.o.) successfully attenuated pro-inflammatory cytokines and NLRP3 inflammasome, and improved cardiac function, heart failure, and survival after MI (myocardial infarction)[7]. Colchicine reduces ALI (acute lung injury) and respiratory failure in experimental ARDS (acute respiratory distress syndrome) in relation with reduced lung neutrophil recruitment and reduced circulating leukocyte activation[8]. | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
2.50mL 0.50mL 0.25mL |
12.52mL 2.50mL 1.25mL |
25.04mL 5.01mL 2.50mL |
| 参考文献 |
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[2]Hastie SB. Interactions of colchicine with tubulin. Pharmacol Ther. 1991;51(3):377-401. [3]Hastie SB. Interactions of colchicine with tubulin. Pharmacol Ther. 1991;51(3):377-401 |