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C29

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Chemical Structure| 363600-92-4 同义名 : TLR2-IN-C29
CAS号 : 363600-92-4
货号 : A134430
分子式 : C16H15NO4
纯度 : 98%
分子量 : 285.295
MDL号 : MFCD01248566
存储条件：

粉末 Inert atmosphere,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 30 mg/mL(105.15 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 C29 acts as an inhibitor of TLR2. It effectively inhibits signaling through human TLR1/2 and TLR2/6 with IC50 values of 19.7μM and 37.6μM, respectively. C29, at concentrations of 10 or 50 μM for 1 hour, dose-dependently obstructs the induction of IL-8 mRNA by P3C and P2C in HEK-TLR2 stable transfectants. Additionally, C29, in the range of 50 to 200 μM for 1 hour, significantly suppresses the expression of the IL-1β gene triggered by P3C and P2C at both 1 hour and 4 hours post-stimulation in THP-1 cells. When treated with C29 at 25 or 50 μM for one hour, there is a significant reduction in TNF-α mRNA and IL-12 p40 protein levels in response to P3C, but not to P2C, in primary murine macrophages. C29, at a concentration of 50 μM for a duration of one hour, inhibits the expression of proinflammatory genes in HEK-TLR2 cells and murine macrophages, which is induced by TLR2 bacterial agonists[1].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.51mL

0.70mL

0.35mL

17.53mL

3.51mL

1.75mL

35.05mL

7.01mL

3.51mL
参考文献

[1]Mistry P, et al. Inhibition of TLR2 signaling by small molecule inhibitors targeting a pocket within the TLR2 TIR domain. Proc Natl Acad Sci U S A. 2015 Apr 28;112(17):5455-60.