产品说明书
Ac-YVAD-cmk
 |
同义名 : | Caspase-1 Inhibitor II;N-Ac-Tyr-Val-Ala-Asp-CMK;N-Ac-Tyr-Val-Ala-Asp-CMK |
| CAS号 : | 178603-78-6 | |
| 货号 : | A1329237 | |
| 分子式 : | C24H33ClN4O8 | |
| 纯度 : | 99%+ | |
| 分子量 : | 540.994 | |
| MDL号 : | MFCD00237124 | |
| 存储条件： |
粉末 Keep in dark place,Inert atmosphere,Store in freezer, under -20°C 液体 -20°C:3-6个月-80°C:12个月 |
|
| 溶解度 : |
DMSO: 105 mg/mL(194.09 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO |
|
| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | Caspase-1 is a key enzyme of the NLRP3 inflammasome, which is a critical pro-inflammatory mediator that modulates host responses to various stress conditions[3]. Ac-YVAD-CMK inhibited activation of M1 microglial and enhanced M2 microglial activation after ICH (Intracerebral hemorrhage)[4]. In mice, Caspase-1 activation was inhibited significantly in a dose-dependent manner by pretreatment with AC-YVAD-CMK (1.25, 6.25, and 12.5 μmol/kg). Furthermore, AC-YVAD-CMK (12.5 μmol/kg) pretreatment significantly reduced the cold-restraint stress induced multiple haemorrhagic gastric erosions and ulcers, as well as the elevated levels of IL-1β, IL-18 and CD68 mRNA expression. Treatment of mice with AC-YVAD-CMK (12.5 μmol/kg) 30 min before cold-stress injury decreased the mortality rate compared with mice that received vehicle. Moreover, AC-YVAD-CMK (12.5 μmol/kg) alleviated the overexpression of phospho (P)-P38 protein and P-IκB induced by cold-restraint stress. In addition, ethanol induced multiple haemorrhagic erosions and ulcers were also alleviated significantly by pretreatment with AC-YVAD-CMK (12.5 μmol/kg)[3]. AC-YVAD-CMK treatment improved rotarod performance time after 72 hrs as compared with ICH group[4]. | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
|
1 mM 5 mM 10 mM |
1.85mL 0.37mL 0.18mL |
9.24mL 1.85mL 0.92mL |
18.48mL 3.70mL 1.85mL |
| 参考文献 |
|---|