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Doxorubicin HCl

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Chemical Structure| 25316-40-9 同义名 : 盐酸阿霉素 ;Hydroxydaunorubicin hydrochloride;Doxorubicin (hydrochloride);DOX;Hydroxydaunorubicin HCl;NSC 123127;Adriamycin;Doxorubicin HCl
CAS号 : 25316-40-9
货号 : A130952
分子式 : C27H30ClNO11
纯度 : 99+%
分子量 : 579.98
MDL号 : MFCD00077757
存储条件：

粉末 Keep in dark place,Inert atmosphere,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 85 mg/mL(146.56 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 50 mg/mL(86.21 mM)，配合低频超声，并水浴加热至45℃助溶
动物实验配方：

PO 0.5% CMC-Na 50 mg/mL suspension

生物活性
描述 Doxorubicin hydrochloride, a potent chemotherapeutic agent and cytotoxic anthracycline antibiotic, inhibits human DNA topoisomerase I and II with IC50 values of 0.8 µM and 2.67 µM, respectively. It diminishes the basal phosphorylation of AMPK and acetyl-CoA carboxylase, leading to the induction of apoptosis and autophagy[1][2][3].
细胞研究
细胞系 浓度 检测类型 检测时间 活动说明 数据源
16HBE 10 μM Growth Inhibition Assay 72 h GI50=0.18 μM 24650643
1A9 Cytotoxic Assay ED50=0.02 μM 17591444
2008/MRP1 Cytotoxic Assay 5 d IC50=0.577 μM 19725578
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.72mL

0.34mL

0.17mL

8.62mL

1.72mL

0.86mL

17.24mL

3.45mL

1.72mL
参考文献

[1]John L. Nitiss, et al. Targeting DNA topoisomerase II in cancer chemotherapy.Nat Rev Cancer. 2009 May;9(5):338-50.

[2]Hee-KyungRhee,et al. Synthesis, cytotoxicity, and DNA topoisomerase II inhibitory activity of benzofuroquinolinediones. Bioorg Med Chem. 2007 Feb 15;15(4):1651-8.

[3]P D Foglesong, et al. Doxorubicin inhibits human DNA topoisomerase I. Cancer Chemother Pharmacol. 1992;30(2):123-5.

[4]Nesstor Pilco-Ferreto, et al. Influence of doxorubicin on apoptosis and oxidative stress in breast cancer cell lines. Int J Oncol. 2016 Aug;49(2):753-62.

[5]Regine Lüpertz, et al. Dose- and time-dependent effects of doxorubicin on cytotoxicity, cell cycle and apoptotic cell death in human colon cancer cells. Toxicology. 2010 May 27;271(3):115-21.

[6]Penelope D Ottewell, et al. Antitumor effects of doxorubicin followed by zoledronic acid in a mouse model of breast cancer. J Natl Cancer Inst. 2008 Aug 20;100(16):1167-78.

[7]Koda LY, Van der Kooy D. Doxorubicin: a fluorescent neurotoxin retrogradely transported in the central nervous system. Neurosci Lett. 1983 Mar 28;36(1):1-8. doi: 10.1016/0304-3940(83)90476-7. PMID: 6190113. 9

[8]Kauffman MK, Kauffman ME, Zhu H, Jia Z, Li YR. Fluorescence-Based Assays for Measuring Doxorubicin in Biological Systems. React Oxyg Species (Apex). 2016;2(6):432-439. doi: 10.20455/ros.2016.873. PMID: 29707647; PMCID: PMC5921830.