Nudifloramide

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Chemical Structure| 701-44-0 同义名 : 2PY;N-methyl-2-Pyridone-5-Carboxamide
CAS号 : 701-44-0
货号 : A130732
分子式 : C7H8N2O2
纯度 : 99%+
分子量 : 152.15
MDL号 : MFCD00457283
存储条件：

Pure form Sealed in dry,Room Temperature

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 60 mg/mL(394.35 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 30 mg/mL(197.17 mM)，配合低频超声助溶
动物实验配方：
生物活性
描述 Poly(ADP-ribose) polymerase (PARP-1) is a nuclear enzyme, highly involved in a variety of extremely important physiologic events, including regulation of gene expression, cellular differentiation, apoptosis, DNA replication, and DNA repair. Moreover, PARP-1 plays an important role in nicotinamide-adenine dinucleotide (NAD) degradation. As one of the end products of NAD degradation, nudifloramide significantly inhibited PARP-1 activity in vitro with an IC50 value of 8 μM (1.22 mg/L). This IC50 value fell within the range of blood nudifloramide concentrations observed in dialysis patients. The nudifloramide concentrations were many times elevated in nondialyzed chronic renal failure (CRF) patients when compared with controls. The direct correlations were found between serum nudifloramide concentrations and serum creatinine concentration, and negative correlations between serum nudifloramide concentrations and creatinine clearance. The concentration of nudifloramide decreased considerably after hemodialysis (HD) session and permanently declined after kidney transplantation suggesting that it could be a novel uremic toxin[2].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

6.57mL

1.31mL

0.66mL

32.86mL

6.57mL

3.29mL

65.72mL

13.14mL

6.57mL
参考文献

[1]Rutkowski B, et al. N-methyl-2-pyridone-5-carboxamide: a novel uremic toxin? Kidney Int Suppl. 2003 May;(84):S19-21.

[2]Lenglet A, Liabeuf S, Bodeau S, Louvet L, Mary A, Boullier A, Lemaire-Hurtel AS, Jonet A, Sonnet P, Kamel S, Massy ZA. N-methyl-2-pyridone-5-carboxamide (2PY)-Major Metabolite of Nicotinamide: An Update on an Old Uremic Toxin. Toxins (Basel). 2016 Nov 15;8(11):339.