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LIN28 inhibitor LI71

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Chemical Structure| 1357248-83-9 同义名 : -
CAS号 : 1357248-83-9
货号 : A1273135
分子式 : C21H21NO3
纯度 : 99%+
分子量 : 335.396
MDL号 : MFCD07363437
存储条件：

粉末 Keep in dark place,Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(313.06 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 LIN28 is an RNA-binding protein that regulates the maturation of the let-7 family of microRNAs by bipartite interactions with let-7 precursors. Through inhibition of let-7 biogenesis, LIN28 functions as a pluripotency factor, as well as a driver of tumorigenesis. LIN28 inhibitor LI71 is a potent and cell-permeable inhibitor of LIN28, which inhibits the interactions between LIN28 and the let-7 family with IC50s of ~7 and ~27 μM in fluorescence polarization assay and in oligouridylation assay, respectively. LI71 could significantly reduce the relative Renilla luciferase activity in cells expressing LIN28A and LIN28B at 50-100 μM. Moreover, treatment of DKO+A cells with 100 μM LI71 for 48 hr increased levels of mature let-7 species compared to vehicle-treated cells[1].
作用机制 LIN28 inhibitor LI71 binds the cold shock domain to suppress LIN28's activity[1].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.98mL

0.60mL

0.30mL

14.91mL

2.98mL

1.49mL

29.82mL

5.96mL

2.98mL
参考文献

[1]Wang L, et al. Small-Molecule Inhibitors Disrupt let-7 Oligouridylation and Release the Selective Blockade of let-7 Processing by LIN28. Cell Rep. 2018 Jun 5;23(10):3091-3101