SAG HCl

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Chemical Structure| 2095432-58-7 同义名 : SAG hydrochloride
CAS号 : 2095432-58-7
货号 : A125368
分子式 : C28H29Cl2N3OS
纯度 : 99%+
分子量 : 526.52
MDL号 : N/A
存储条件：

Pure form Inert atmosphere, 2-8°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 20 mg/mL(37.99 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 25 mg/mL(47.48 mM)，配合低频超声助溶
动物实验配方：
生物活性
描述 SAG hydrochloride is a highly effective agonist of the Smoothened (Smo) receptor (EC50=3 nM; KD=59 nM). It activates the Hedgehog signaling pathway and antagonizes the inhibitory effects of Cyclopamine on Smo[1][2][3].
细胞研究
细胞系 浓度 检测类型 检测时间 活性说明 数据源
HUVEC Cytotoxicity assay 24 h Cytotoxicity against HUVEC after 24 hrs by WST-1 assay, IC50=22 μM 22985958
mouse Shh-Light II cell Function assay 48 hrs Activation of Shh pathway in mouse Shh-Light II cells assessed as induction of gli1 expression after 48 hrs by renilla luminescence assay, EC50=0.0088 μM 22985958
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.90mL

0.38mL

0.19mL

9.50mL

1.90mL

0.95mL

18.99mL

3.80mL

1.90mL
参考文献

[1]Chen JK, et al. Small molecule modulation of Smoothened activity. Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14071-6.

[2]Stanton BZ, et al. A small molecule that binds Hedgehog and blocks its signaling in human cells. Nat Chem Biol. 2009 Mar;5(3):154-6.

[3]Guerrini G, et, al. Inhibition of smoothened in breast cancer cells reduces CAXII expression and cell migration. J Cell Physiol. 2018 Dec; 233(12): 9799-9811.

[4]Lee S, et al. Combining Smoothened Agonist (SAG) and NEL-like protein-1 (NELL-1) Enhances Bone Healing. Plast Reconstr Surg. 2017 Feb 13.