产品说明书

GRL0617

Print
Chemical Structure| 1093070-16-6 同义名 : -
CAS号 : 1093070-16-6
货号 : A1251839
分子式 : C20H20N2O
纯度 : 99%+
分子量 : 304.386
MDL号 : MFCD12547683
存储条件：

粉末 Keep in dark place,Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 120 mg/mL(394.24 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 The papain-like protease (PLpro) is a viable target for the development of antivirals against coronavirus-caused severe acute respiratory syndrome (SARS-CoV). GRL0617 is a potent competitive inhibitor of PLpro with a Ki value of 0.49 ± 0.08μM. The IC50 value of GRL0617 for PLpro activity in vitro is 0.6 ± 0.1μM. The antiviral EC50 value of GRL0617 against SARS-CoV is 14.5 ± 0.8μM. GRL0617 is unlikely to inhibit other human ubiquitin-specific proteases, such as HAUSP, USP18, UCH-L1, UCH-L3, and papain-like protease (IC50 > 100μM)[1]. The in vitro IC50 value of GRL0617 against SARS-CoV-2 PLpro is 2.1 ± 0.2μM[2].
作用机制 GRL0617 binds within the S3 and S4 subsites of PLpro. The interaction between them is stabilized through a pair of hydrogen bonds and a series of hydrophobic interactions[1].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.29mL

0.66mL

0.33mL

16.43mL

3.29mL

1.64mL

32.85mL

6.57mL

3.29mL
参考文献

[1]Ratia K, Pegan S, Takayama J, Sleeman K, Coughlin M, Baliji S, Chaudhuri R, Fu W, Prabhakar BS, Johnson ME, Baker SC, Ghosh AK, Mesecar AD. A noncovalent class of papain-like protease/deubiquitinase inhibitors blocks SARS virus replication. Proc Natl Acad Sci U S A. 2008 Oct 21;105(42):16119-24

[2]Fu Z, Huang B, Tang J, Liu S, Liu M, Ye Y, Liu Z, Xiong Y, Zhu W, Cao D, Li J, Niu X, Zhou H, Zhao YJ, Zhang G, Huang H. The complex structure of GRL0617 and SARS-CoV-2 PLpro reveals a hot spot for antiviral drug discovery. Nat Commun. 2021 Jan 20;12(1):488