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Camalexin

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Chemical Structure| 135531-86-1 同义名 : -
CAS号 : 135531-86-1
货号 : A1209961
分子式 : C11H8N2S
纯度 : 99%+
分子量 : 200.26
MDL号 : MFCD18451889
存储条件：

粉末 Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 120 mg/mL(599.22 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Camalexin is a phytoalexin isolated from Camelina sativa and Arabidopsis (Cruciferae) with antibacterial, antifungal, antiproliferative and anticancer activities. Camalexin can induce reactive oxygen species (ROS) production. ROS levels, SOD activity, CAT activity, and GSSG levels were significantly enhanced while the GSH level was decreased after treatment of camalexin. Administration of camalexin suppresses xenograft tumor graft growth without obvious toxicity[1]. Camalexin triggers cell shrinkage and phospholipid scrambling of the erythrocyte cell membrane, an effect at least in part depending on Ca2+ entry, as well as staurosporine and chelerythrine sensitive kinase(s) as well as zVAD and zIETD-fmk sensitive caspase(s)[2]. Camalexin reduces cell viability via CD (cathepsin D) and may present as a novel therapeutic agent for treatment of metastatic prostate cancer cells[3]. Treatment of Jurkat cells with camalexin resulted in activation of caspase-8, caspase-9, caspases-3/7, and apoptosis that was detected by the presence of a sub-G1 population of cells, externalization of phosphatidyl serine and decreased mitochondrial membrane potential[4]. Camalexin increased ROS, decreased cell proliferation, and increased apoptosis more significantly in C4-2 and ARCaP-Snail cells as compared to LNCaP and ARCaP-Neo cells, respectively, while normal prostate epithelial cells (PrEC) were unaffected. The ROS scavenger N-acetyl cysteine (NAC) antagonized the effects of camalexin, whereas the addition of exogenous hydrogen peroxide potentiated the effects of camalexin, showing that camalexin is mediating its effects through ROS[5].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

4.99mL

1.00mL

0.50mL

24.97mL

4.99mL

2.50mL

49.94mL

9.99mL

4.99mL
参考文献

[1]Yang Y, Wang G, Wu W, Yao S, Han X, He D, He J, Zheng G, Zhao Y, Cai Z, Yu R. Camalexin Induces Apoptosis via the ROS-ER Stress-Mitochondrial Apoptosis Pathway in AML Cells. Oxid Med Cell Longev. 2018 Nov 14;2018:7426950

[2]Almasry M, Jemaà M, Mischitelli M, Lang F, Faggio C. Camalexin-Induced Cell Membrane Scrambling and Cell Shrinkage in Human Erythrocytes. Cell Physiol Biochem. 2017;41(2):731-741

[3]Smith B, Randle D, Mezencev R, Thomas L, Hinton C, Odero-Marah V. Camalexin-induced apoptosis in prostate cancer cells involves alterations of expression and activity of lysosomal protease cathepsin D. Molecules. 2014 Apr 2;19(4):3988-4005

[4]Mezencev R, Updegrove T, Kutschy P, Repovská M, McDonald JF. Camalexin induces apoptosis in T-leukemia Jurkat cells by increased concentration of reactive oxygen species and activation of caspase-8 and caspase-9. J Nat Med. 2011 Jul;65(3-4):488-99

[5]Smith BA, Neal CL, Chetram M, Vo B, Mezencev R, Hinton C, Odero-Marah VA. The phytoalexin camalexin mediates cytotoxicity towards aggressive prostate cancer cells via reactive oxygen species. J Nat Med. 2013 Jul;67(3):607-18