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Biochanin A

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Chemical Structure| 491-80-5 同义名 : 4-Methylgenistein;Olmelin;Pratensol;Biochanin;NSC 123538;5,7-dihydroxy-4'-Methoxyisoflavone;4’-methyl Genistein
CAS号 : 491-80-5
货号 : A120547
分子式 : C16H12O5
纯度 : 98+%
分子量 : 284.264
MDL号 : MFCD00006839
存储条件：

粉末 Sealed in dry,Room Temperature

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 50 mg/mL(175.89 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
靶点

  • FAAH

    FAAH (mouse), IC50:1.8 μM

    FAAH (human), IC50:1.4 μM
描述 Anandamide (arachidonoylethanolamide, AEA) is endogenous ligands of cannabinoid CB1 and CB2 receptors and AEA is metabolized by the enzyme fatty acid amide hydrolase (FAAH). Biochanin A (4-Methylgenistein; Olmelin), a major isoflavone in red clover, is a naturally occurring FAAH inhibitor, with IC50 values of 1.8, 1.4 and 2.4 µM for mouse, rat and human FAAH ,respectively[3]. In vitro, Biochanin A inhibited human osteosarcoma cell growth and colony formation in a dose-dependent manner, and induced apoptosis by triggering activation of the intrinsic mitochondrial pathway and caspase-9 and -3[4]. In vivo, type 2 diabetes mellitus animals treated with 10, 20 and 40 mg/kg of Biochanin A for 28 days showed significantly decreased blood glucose, reduced insulin resistance and improved inulin sensitivity, compared to model group. These effects might be linked to its effects on SIRT1[5].
临床研究
NCT号 适应症或疾病 临床期 招募状态 预计完成时间 地点
NCT02174666 Osteopenia Os... 展开 >>teoporosis 收起 << Not Applicable Unknown September 2016 Denmark ... 展开 >> Aarhus University Hospital Aarhus, Central Jutland Region, Denmark, 8000 收起 <<
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.52mL

0.70mL

0.35mL

17.59mL

3.52mL

1.76mL

35.18mL

7.04mL

3.52mL
参考文献

[1]Thors L, Burston JJ, et al. Biochanin A, a naturally occurring inhibitor of fatty acid amide hydrolase. Br J Pharmacol. 2010 Jun;160(3):549-60.

[2]Akiyama T, Ishida J, et al. Genistein, a specific inhibitor of tyrosine-specific protein kinases. J Biol Chem. 1987 Apr 25;262(12):5592-5.

[3]Thors L, Burston JJ, Alter BJ, McKinney MK, Cravatt BF, Ross RA, Pertwee RG, Gereau RW 4th, Wiley JL, Fowler CJ. Biochanin A, a naturally occurring inhibitor of fatty acid amide hydrolase. Br J Pharmacol. 2010 Jun;160(3):549-60. doi: 10.1111/j.1476-5381.2010.00716.x. PMID: 20590565; PMCID: PMC2931556.

[4]Hsu YN, Shyu HW, Hu TW, Yeh JP, Lin YW, Lee LY, Yeh YT, Dai HY, Perng DS, Su SH, Huang YH, Su SJ. Anti-proliferative activity of biochanin A in human osteosarcoma cells via mitochondrial-involved apoptosis. Food Chem Toxicol. 2018 Feb;112:194-204. doi: 10.1016/j.fct.2017.12.062. Epub 2018 Jan 3. PMID: 29305928.

[5]Oza MJ, Kulkarni YA. Biochanin A improves insulin sensitivity and controls hyperglycemia in type 2 diabetes. Biomed Pharmacother. 2018 Nov;107:1119-1127. doi: 10.1016/j.biopha.2018.08.073. Epub 2018 Aug 27. PMID: 30257324.