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AN1

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Chemical Structure| 486443-73-6 同义名 : LC3-mHTT-IN-AN1
CAS号 : 486443-73-6
货号 : A1193421
分子式 : C15H9Br2NO3
纯度 : 98%+
分子量 : 411.045
MDL号 : MFCD03235520
存储条件：

粉末 Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 120 mg/mL(291.94 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Mutant huntingtin protein (mHTT) contains an expanded polyglutamine tract and causes Huntington’s disease (HD). Microtubule-associated proteins 1A/1B light chain 3B (LC3) is a protein associated with autophagy substrate selection and autophagosome biogenesis. mHTT can be degraded by autophagy, during which protein substrates are incorporated into autophagosomes associated with LC3. LC3-mHTT-IN-AN1 is a mHTT-LC3 linker compound that interacts with both mHTT and LC3, but not with wild-type HTT. LC3-mHTT-IN-AN1 at concentrations of 10 – 300nM reduced the level of mHTT in cultured HD mouse neurons in an allele-selective manner. LC3-mHTT-IN-AN1 also reduced the level of mutant but not wild-type ATXN3 in primary fibroblasts from patients with spinocerebellar ataxia type 3. Treatment with LC3-mHTT-IN-AN1 (2 μl at 25 μM per day) for 10 days led to a significant lowering of mHTT in the cortices of HD mice[1].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.43mL

0.49mL

0.24mL

12.16mL

2.43mL

1.22mL

24.33mL

4.87mL

2.43mL
参考文献

[1]Li Z, Wang C, Wang Z, Zhu C, Li J, Sha T, Ma L, Gao C, Yang Y, Sun Y, Wang J, Sun X, Lu C, Difiglia M, Mei Y, Ding C, Luo S, Dang Y, Ding Y, Fei Y, Lu B. Allele-selective lowering of mutant HTT protein by HTT-LC3 linker compounds. Nature. 2019 Nov;575(7781):203-209. doi: 10.1038/s41586-019-1722-1