KU-57788
产品说明书
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同义名 : | NU7441 |
| CAS号 : | 503468-95-9 | |
| 货号 : | A118825 | |
| 分子式 : | C25H19NO3S | |
| 纯度 : | 99%+ | |
| 分子量 : | 413.488 | |
| MDL号 : | MFCD11983069 | |
| 存储条件： |
Pure form Sealed in dry,Store in freezer, under -20°C In solvent -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
DMSO: 15 mg/mL(36.28 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO |
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| 动物实验配方： |
IP 2% DMSO+water 0.2 mg/mL clear PO 0.5% CMC-Na 48 mg/mL suspension |
| 生物活性 | |||
|---|---|---|---|
| 靶点 |
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| 描述 | NU7441 increases the persistence of γH2AX foci after ionizing radiation–induced or etoposide-induced DNA damage. NU7441 (0.5 μM or 1 μM) appreciably increases G2-M accumulation induced by ionizing radiation, etoposide, and doxorubicin in both SW620 and LoVo cells. NU7441 causes persistence of doxorubicin- and ionising radiation-induced DNA double-strand break and also slightly decreases homologous recombination activity DNA-PK-proficient M059-Fus-1 and DNA-PK-deficient M059 J human tumour cells. NU7441 inhibits UV-induced RPA p34 hyperphosphorylation in a dose-dependent manner both in cells lacking and cells expressing polymerase η. NU7441 increases levels of fludarabine-induced γH2AX foci and correspondingly decreased fludarabine-induced cell death in chronic lymphocytic leukemia cells. NU7441 also inhibits mitoxantrone-induced DNA-PKcs autophosphorylation and repair in chronic lymphocytic leukemia cells. It reduces the frequency of NHEJ while increasing the rate of HDR following Cas9-mediated DNA cleavage. | ||
| 细胞研究 | |||||
|---|---|---|---|---|---|
| 细胞系 | 浓度 | 检测类型 | 检测时间 | 活动说明 | 数据源 |
| 23132-87 | Growth Inhibition Assay | IC50=5.32024 μM | SANGER | ||
| 639-V | Growth Inhibition Assay | IC50=1.44128 μM | SANGER | ||
| 647-V | Growth Inhibition Assay | IC50=15.2705 μM | SANGER | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
2.42mL 0.48mL 0.24mL |
12.09mL 2.42mL 1.21mL |
24.18mL 4.84mL 2.42mL |
