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ML367

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Chemical Structure| 381168-77-0 同义名 : -
CAS号 : 381168-77-0
货号 : A1177117
分子式 : C19H12F2N4
纯度 : 99%+
分子量 : 334.322
MDL号 : MFCD01897460
存储条件：

粉末 Keep in dark place,Inert atmosphere,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 120 mg/mL(358.94 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 ATPase family AAA domain-containing protein 5 (ATAD5) is a tumor suppressor responsible for the maintenance of genomic stability and the inhibition of tumorigenesis. ML367 is a probe molecular that inhibits ATAD5 stabilization with an IC50 value of 1.2μM. In cells deficient in DNA damage repair proteins, such as PARP1, Lig3, Lig4, FancM, FancG, and Rad54b, treatment with ML367 significantly inhibited cell growth in colony formation assays. In HEK293T cells transfected with FLAG-tagged ATAD5, treatment with 20 μM 5-FUrd resulted in an increase in ATAD5 expression as compared to DMSO-treated controls, whereas ML367 at the concentrations of 5 – 40μM inhibited the elevated protein expression of ATAD5 in 5-FUrd-treated cells. The microsomal stability (T1/2) of ML367 in rats is 11min. The stability of ML367 to human liver microsomes is 57.9% after 30min. The mouse plasma stability (T1/2) of ML367 is >90min.
作用机制 ML367 is an inhibitor of ATAD5 stabilization that blocks UV irradiation-induced DNA damage responses, including CHK1-phosphorylation and RPA32-phosphorylation.
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.99mL

0.60mL

0.30mL

14.96mL

2.99mL

1.50mL

29.91mL

5.98mL

2.99mL