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Spastazoline

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Chemical Structure| 2351882-11-4 同义名 : -
CAS号 : 2351882-11-4
货号 : A1176737
分子式 : C20H30N8
纯度 : 99%+
分子量 : 382.506
MDL号 : MFCD31813810
存储条件：

粉末 Keep in dark place,Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(274.51 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 The AAA superfamily (ATPases associated with diverse cellular activities) has been linked to a wide range of cellular processes, including cell division, cytoskeleton organization, and organelle biogenesis. Spastin is a microtubule-severing AAA protein needed for cell division and intracellular vesicle transport. Chemical probes that inhibit their activities in cells on similarly fast timescales can be valuable tools to dissect dynamic mechanisms. Spastazoline is a potent and selective cell-permeable probe for spastin with an IC50 of 99 ± 18 nM for human spastin. Treating HeLa-WT cells with spastazoline for 4.5 hours (10 µM) resulted in a ~2-fold increase in the number of cells with intercellular bridges compared to DMSO control indicating that spastazoline inhibits intercellular bridge disassembly by interfering with spastin activity. In dividing HeLa-WT cells treated with Spastazoline, EGFP-spastin accumulated at chromatin within 2–4 min after cleavage furrow ingression initiation. Imaging HeLa-WT cells in anaphase treated with spastazoline (10 µM for 1 hour) also revealed GFP-spastin puncta on chromosomes[1].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.61mL

0.52mL

0.26mL

13.07mL

2.61mL

1.31mL

26.14mL

5.23mL

2.61mL
参考文献

[1]Cupido T, Pisa R, Kelley ME, Kapoor TM. Designing a chemical inhibitor for the AAA protein spastin using active site mutations. Nat Chem Biol. 2019;15(5):444-452