SSE15206

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Chemical Structure| 1370046-40-4 同义名 : -
CAS号 : 1370046-40-4
货号 : A1176717
分子式 : C19H21N3O3S
纯度 : 99%+
分子量 : 371.453
MDL号 : MFCD31746881
存储条件：

粉末 Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 145 mg/mL(390.36 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Microtubules are highly dynamic structures that form spindle fibres during mitosis and are one of the most validated cancer targets. SSE15206 is a microtubule polymerization inhibitor with GI50 value of 197 nM in HCT116 cells. SSE15206 also exhibited potent antiproliferative activities against several other cancer cell lines of different origins (leukaemia, breast, lung, and cervical) with sub-micromolar GI50 values. Further, dose-dependent increase in phosphorylation of both histone H3 and MPM2 was observed in CT116 cells following treatment with SSE15206 (0.5 μM, 1 μM and 2 μM) for 4 and 8 hours indicating mitotic arrest. Similarly, treatment with SSE15206 for 8 and 24 hours increased mitotic index (percentage mitotic cells) compared to the DMSO control. Within four hours of treatment with 1 μM SSE15206, CT116 cells started accumulating in the G2/M phase of the cell cycle compared to the DMSO control. The G2/M arrest increased in a time-dependent manner and peaked at 12 hours. Moreover, treatment of three different cell types, HCT116, A549 and CAL-51 with SSE15206 (0.5 μM, 1 μM and 2 μM) for 24 hours, resulted in increased expression of p53 and p21 compared to DMSO control[1].
作用机制 SSE15206 inhibits microtubule polymerization by binding to colchicine site in tubulin[1].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.69mL

0.54mL

0.27mL

13.46mL

2.69mL

1.35mL

26.92mL

5.38mL

2.69mL
参考文献

[1]Manzoor S, Bilal A, Khan S, et al. Identification and characterization of SSE15206, a microtubule depolymerizing agent that overcomes multidrug resistance. Sci Rep. 2018;8(1):3305