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5-FAM

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Chemical Structure| 76823-03-5 同义名 : 5-Carboxyfluorescein
CAS号 : 76823-03-5
货号 : A117509
分子式 : C21H12O7
纯度 : 97%
分子量 : 376.316
MDL号 : MFCD00036874
存储条件：

粉末 Keep in dark place,Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 30 mg/mL(79.72 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：

IP 2% DMSO+2% Tween80+40% PEG300+water 2 mg/mL clear

PO 0.5% CMC-Na 24 mg/mL suspension

生物活性
描述 5-FAM (5-Carboxyfluorescein) is a green fluorescent reagent used for in situ labeling peptides, proteins and nucleotides. 5-FAM is a single isomer with Ex/Em of 490 nm/520 nm. 5-FAM contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition to its relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility, fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser[1]. The combination of 5-Fam as fluorophore and Dabcyl as quencher resulted in a triple-helical substrate that, compared with the fluorophore/quencher pair of Mca/Dnp, had a slightly destabilized triple-helix but was hydrolyzed more rapidly by MMP-1 and MMP-13 and had greater sensitivity[2]. Folic acid (FA) and 5-carboxylic acid fluorescein (5-FAM) were used to modify Fe-MIL-101 to construct a functionalized nano-platform (5-FAM/FA/TP@Fe-MIL-101) for the targeted delivery of the anti-tumor drug triptolide and realize in vivo fluorescence imaging. Compared with Fe-MIL-101, functionalized nanoparticles not only showed better targeted therapy efficiency, but also reduced the systemic toxicity of triptolide. In addition, the modification of 5-FAM facilitated fluorescence imaging of the tumor site and realized the construction of an integrated nano-platform for fluorescence imaging and treatment[3]. Using 5'-FAM-labeled primer and esterified dNTP with a dye to show that the reaction can proceed to ca. 450 base pairs, and that the intermediates of many individual steps can be identified[4].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.66mL

0.53mL

0.27mL

13.29mL

2.66mL

1.33mL

26.57mL

5.31mL

2.66mL
参考文献

[1]Lee, J., Suganya, AA., Song, JM. Peptide substrate-based inkjet printing high-throughput MMP-9 anticancer assay using fluorescence resonance energy transfer (FRET). ELSEVIER. 2018; 256: 1093-1099.

[2]Tokmina-Roszyk M, Tokmina-Roszyk D, Fields GB. The synthesis and application of Fmoc-Lys(5-Fam) building blocks. Biopolymers. 2013 Jul;100(4):347-55

[3]Cai M, Zeng Y, Liu M, You L, Huang H, Hao Y, Yin X, Qu C, Ni J, Dong X. Construction of a Multifunctional Nano-Scale Metal-Organic Framework-Based Drug Delivery System for Targeted Cancer Therapy. Pharmaceutics. 2021 Nov 17;13(11):1945

[4]LinWu SW, Tu YH, Tsai TY, Maestre-Reyna M, Liu MS, Wu WJ, Huang JY, Chi HW, Chang WH, Chiou CF, Wang AH, Lee J, Tsai MD. Thermococcus sp. 9°N DNA polymerase exhibits 3'-esterase activity that can be harnessed for DNA sequencing. Commun Biol. 2019 Jun 20;2:224