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GS-441524

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Chemical Structure| 1191237-69-0 同义名 : -
CAS号 : 1191237-69-0
货号 : A1159246
分子式 : C12H13N5O4
纯度 : 99%
分子量 : 291.263
MDL号 : MFCD32666994
存储条件：

粉末 Keep in dark place,Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 85 mg/mL(291.83 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 GS-441524 is an antiviral nucleoside analog with an EC50 value of 11.1μM for delayed brain tumor cells infected with murine hepatitis virus. The mean EC50 value of GS-441524 for SARS-CoV- and MERS-CoV-infected primary human airway epithelial cells is approximately 0.86μM[1]. In Calu3 2B4 cells, GS-441524 inhibited SARS-CoV-2 replication with EC50 and EC90 values of 0.62μM and 1.34μM, respectively, as determined by infectious viral titer. The EC50 and EC90 values of GS-441524 for SARS-CoV-2 replication in Calu3 2B4 cells were 1.09μM and 1.37μM. respectively, as measured by qRT-PCR. In Vero E6 cells, the EC50 value of GS-441524 for SARS-CoV-2 replication was 0.47μM as determined by both infectious viral titer and qRT-PCR[2].
作用机制 GS-441524 is a 1’-cyano 4-aza-7,9-dideazaadenosine C-adenosine nucleoside analogue that inhibits multiple virus families in vitro[1].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.43mL

0.69mL

0.34mL

17.17mL

3.43mL

1.72mL

34.33mL

6.87mL

3.43mL
参考文献

[1]Agostini ML, Andres EL, Sims AC, Graham RL, Sheahan TP, Lu X, Smith EC, Case JB, Feng JY, Jordan R, Ray AS, Cihlar T, Siegel D, Mackman RL, Clarke MO, Baric RS, Denison MR. Coronavirus Susceptibility to the Antiviral Remdesivir (GS-5734) Is Mediated by the Viral Polymerase and the Proofreading Exoribonuclease. mBio. 2018 Mar 6;9(2):e00221-18. doi: 10.1128/mBio.00221-18

[2]Pruijssers AJ, George AS, Schäfer A, Leist SR, Gralinksi LE, Dinnon KH, Yount BL, Agostini ML, Stevens LJ, Chappell JD, Lu X, Hughes TM, Gully K, Martinez DR, Brown AJ, Graham RL, Perry JK, Du Pont V, Pitts J, Ma B, Babusis D, Murakami E, Feng JY, Bilello JP, Porter DP, Cihlar T, Baric RS, Denison MR, Sheahan TP. Remdesivir potently inhibits SARS-CoV-2 in human lung cells and chimeric SARS-CoV expressing the SARS-CoV-2 RNA polymerase in mice. bioRxiv [Preprint]. 2020 Apr 27:2020.04.27.064279. doi: 10.1101/2020.04.27.064279