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AUDA

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Chemical Structure| 479413-70-2 同义名 : -
CAS号 : 479413-70-2
货号 : A1148766
分子式 : C23H40N2O3
纯度 : 98%+
分子量 : 392.575
MDL号 : MFCD12912267
存储条件：

粉末 Sealed in dry,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 120 mg/mL(305.67 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 The soluble epoxide hydrolase (sEH) is involved in the metabolism of arachidonic, linoleic, and other fatty acid epoxides, endogenous chemical mediators that play an important role in blood pressure regulation and inflammation. AUDA is a potent sEH inhibitor with IC50s of 18±1 and 69±2 nM for the mouse and human sEH, respectively[1]. AUDA (0.3 to 10 μg/mL) dose-dependently suppressed the proliferation of rat VSMCs (vascular smooth muscle cells) exposed to PDGF (platelet-derived growth factor) for 48 h. AUDA (1–30 μg/mL) dose-dependently inhibited the protein expression of Pin1 and increased HO-1 (heme oxygenase-1) protein levels in PDGF-treated VSMCs at a dose of 3 μg/mL, although potent induction was seen only at 30 μg/mL. PDGF increased COX-2 protein levels in rat VSMCs, and AUDA (1–30 μg/mL; 30 min) dose-dependently upregulated COX-2 expression[2]. Furthermore, in the mouse model of Kawasaki disease (KD), AUDA reduced the protein expression of MMP-9, IL-1β and TNF-α, indicating that AUDA may alleviate inflammatory reactions in the coronary arteries of KD model mice[3].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.55mL

0.51mL

0.25mL

12.74mL

2.55mL

1.27mL

25.47mL

5.09mL

2.55mL
参考文献

[1]Morisseau C, Goodrow MH, Newman JW, Wheelock CE, Dowdy DL, Hammock BD. Structural refinement of inhibitors of urea-based soluble epoxide hydrolases. Biochem Pharmacol. 2002;63(9):1599-1608

[2]Kim HS, Kim SK, Kang KW. Differential Effects of sEH Inhibitors on the Proliferation and Migration of Vascular Smooth Muscle Cells. Int J Mol Sci. 2017;18(12):2683

[3]Dai N, Zhao C, Kong Q, Li D, Cai Z, Wang M. Vascular repair and anti-inflammatory effects of soluble epoxide hydrolase inhibitor. Exp Ther Med. 2019;17(5):3580-3588