产品说明书
Napabucasin
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同义名 : | BBI608 |
| CAS号 : | 83280-65-3 | |
| 货号 : | A113651 | |
| 分子式 : | C14H8O4 | |
| 纯度 : | 98% | |
| 分子量 : | 240.211 | |
| MDL号 : | MFCD28155270 | |
| 存储条件： |
粉末 Sealed in dry,Store in freezer, under -20°C 液体 -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
DMSO: 4 mg/mL(16.65 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO |
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| 动物实验配方： |
5% DMSO+40% PEG 300+5% Tween 80+water 1 mg/mL |
| 生物活性 | |||
|---|---|---|---|
| 靶点 |
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| 描述 | Napabucasin targets stemness markers, effectively killing stemness-high cancer cells from various tumors, except in prostate cancer (PCa). It inhibits PCa cell proliferation, motility, survival, and colony formation, while increasing apoptosis and sensitivity to docetaxel. Napabucasin also blocks sphere formation and kills prostate cancer stem cells (PrCSCs) and suppresses stemness gene expression. In PC-3 and 22RV1 cells, it reduces proliferation significantly from day 2 to 5 compared to controls (P<0.05) [1]. | ||
| 细胞研究 | |||||
|---|---|---|---|---|---|
| 细胞系 | 浓度 | 检测类型 | 检测时间 | 活动说明 | 数据源 |
| human DU145 cells | Function assay | 72 h | Anticancer activity against human DU145 cells after 72 hrs by XTT assay, IC50=0.02304 μM | 18829316 | |
| human HaCaT cells | Function assay | 48 h | Antihyperproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopy, IC50=0.5 μM | 22845014 | |
| human HaCaT cells | Proliferation assay | Antiproliferative activity against human HaCaT cells, IC50=0.5 μM | 10479319 | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
4.16mL 0.83mL 0.42mL |
20.82mL 4.16mL 2.08mL |
41.63mL 8.33mL 4.16mL |
| 参考文献 |
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