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(-)-Epigallocatechin Gallate

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Chemical Structure| 989-51-5 同义名 : (-)-表没食子儿茶素没食子酸酯 ;EGCG;Epigallocatechol Gallate;EGCG-Epigallocatechin Gallate)
CAS号 : 989-51-5
货号 : A110153
分子式 : C22H18O11
纯度 : 99%
分子量 : 458.372
MDL号 : -
存储条件：

粉末 Keep in dark place,Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 30 mg/mL(65.45 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 20 mg/mL(43.63 mM)，配合低频超声助溶
动物实验配方：
生物活性
靶点

  • EGFR/ErbB1
描述 Epigallocatechin-3-gallate (EGCG), also termed Epigallocatechol Gallate, the most abundant and most biologically active catechin from green tea, is a potential therapeutic agent for preventing neurodegenerative, inflammatory diseases and cancer. In vitro EGCG supplementation dose-dependently reduced neutrophil migration induced by chemokine interleukin (IL)-8 with an IC50 lower than 1 mM and by cytokine-induced neutrophil chemoattractant-1 at a dose of 15 mg/ml or higher[3]. PC12 cells cultured without serum were administrated with EGCG (0.1 and 1 μM) for 24h which resulted in improved cell morphology and promoted neurite outgrowth, and significant lower cell mortality as compared with serum free group (~40%). There was a gradual increase in neurite length after exposure to EGCG (0.1 and 1 μM); at 4 days of EGCG (1 μM) treatment 40 ± 0.9 and 25.2 ± 2.2% of detected cells exhibited extended neurites, respectively[4]. EGCG decreased cell viability as concentration- and time-dependently, with IC50 values of 14.17 µM for human breast cancer cell line T47D and 193.10 µM for normal human foreskin fibroblast cell line HFF cells, through down-regulation of PI3K/AKT and telomerase[5].
细胞研究
细胞系 浓度 检测类型 检测时间 活动说明 数据源
CHO cells Cytotoxic assay 48 h Cytotoxicity against CHO cells expressing OATP1B3 haplotype 1 after 48 hrs by fluorescence based CellTiter-Glo assay, IC50=3.2 μM 23327877
HSC-T6 cells Function assay 48 h Antifibrotic activity against rat HSC-T6 cells assessed as inhibition of proliferation after 48 hrs by BrdU incorporation assay, IC50=9.9 μM 21504848
HSC-T6 cells Proliferation assay Antiproliferative activity against rat HSC-T6 cells assessed as reduction in cell viability, IC50=29.8 μM 25322455
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.18mL

0.44mL

0.22mL

10.91mL

2.18mL

1.09mL

21.82mL

4.36mL

2.18mL
参考文献

[1]Pae M, Wu D. Immunomodulating effects of epigallocatechin-3-gallate from green tea: mechanisms and applications. Food Funct. 2013 Sep;4(9):1287-303.

[2]Khan N, Afaq F, et al. Targeting multiple signaling pathways by green tea polyphenol (-)-epigallocatechin-3-gallate. Cancer Res. 2006 Mar 1;66(5):2500-5.

[3]Donà M, Dell'Aica I, Calabrese F, Benelli R, Morini M, Albini A, Garbisa S. Neutrophil restraint by green tea: inhibition of inflammation, associated angiogenesis, and pulmonary fibrosis. J Immunol. 2003 Apr 15;170(8):4335-41. doi: 10.4049/jimmunol.170.8.4335. PMID: 12682270.

[4]Reznichenko L, Amit T, Youdim MB, Mandel S. Green tea polyphenol (-)-epigallocatechin-3-gallate induces neurorescue of long-term serum-deprived PC12 cells and promotes neurite outgrowth. J Neurochem. 2005 Jun;93(5):1157-67. doi: 10.1111/j.1471-4159.2005.03085.x. PMID: 15934936.

[5]Moradzadeh M, Hosseini A, Erfanian S, Rezaei H. Epigallocatechin-3-gallate promotes apoptosis in human breast cancer T47D cells through down-regulation of PI3K/AKT and Telomerase. Pharmacol Rep. 2017 Oct;69(5):924-928. doi: 10.1016/j.pharep.2017.04.008. Epub 2017 Apr 13. PMID: 28646740.