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GANT61

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Chemical Structure| 500579-04-4 同义名 : -
CAS号 : 500579-04-4
货号 : A109328
分子式 : C27H35N5
纯度 : 98%
分子量 : 429.6
MDL号 : MFCD14635408
存储条件：

粉末 Sealed in dry,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 25 mg/mL(58.19 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

无水乙醇: 60 mg/mL(139.66 mM)，配合低频超声助溶，注意：无水乙醇开封后，易挥发，也会吸收空气中的水分，导致溶解能力下降，请避免使用开封较久的乙醇
动物实验配方：
生物活性
描述 GANT61 (20 μM) induces more significant cell death compared to targeting Smo (cyclopamine). It also inhibits the clonogenic survival of human colon carcinoma cell lines (0, 5, 10, 20 μM). Moreover, GANT61 (20 μM, 0-72 hr) downregulates Gli1 and Gli2 expression in HT29 cells. Additionally, GANT61 (0, 10 μM, or 20 μM) differentially regulates genes involved in the balance between cell death and survival[1]. GANT-61 inhibits cell viability and induces apoptosis in pancreatic CSCs. It also suppresses the expression of downstream targets of the Shh pathway, decreases Gli-DNA interaction, Gli transcriptional activity, and Gli nuclear translocation in pancreatic CSCs. Furthermore, GANT-61 modulates genes associated with cell survival, cell death, and pluripotency, while also inhibiting the motility, invasion, and migration of CSCs[2]. The sensitivity of GANT61 correlates positively with GLI1 and negatively with MYCN expression in the tested neuroblastoma cell lines. GANT61 downregulates the expression of GLI1, c-MYC, MYCN, and Cyclin D1, and induces apoptosis in neuroblastoma cells[3].
细胞研究
细胞系 浓度 检测类型 检测时间 活动说明 数据源
HEK293 cells Function assay Reduction of GLI2 mediated transcription in HEK293 cells by luciferase reporter assay 17494766
HEK293 cells Function assay Inhibition of beta galactosidase in HEK293 cells 17494766
human 22Rv cells Function assay Reduction of expression of PTCH mRNA in human 22Rv cells 17494766
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.33mL

0.47mL

0.23mL

11.64mL

2.33mL

1.16mL

23.28mL

4.66mL

2.33mL
参考文献

[1]Mazumdar T, et al. Hedgehog signaling drives cellular survival in human colon carcinoma cells. Cancer Res. 2011 Feb 1;71(3):1092-102.

[2]Fu J, et al. GANT-61 inhibits pancreatic cancer stem cell growth in vitro and in NOD/SCID/IL2R gamma null mice xenograft. Cancer Lett. 2013 Mar 1;330(1):22-32.

[3]Wickstrom M, et al. Targeting the hedgehog signal transduction pathway at the level of GLI inhibits neuroblastoma cell growth in vitro and in vivo. Int J Cancer. 2013 Apr 1;132(7):1516-24.