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Maackiain

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Chemical Structure| 19908-48-6 同义名 : DL-​Maackiain;Demethylpterocarpin;Inermin
CAS号 : 19908-48-6
货号 : A108101
分子式 : C16H12O5
纯度 : 99%+
分子量 : 284.264
MDL号 : MFCD00270457
存储条件：

粉末 Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 250 mg/mL(879.47 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 DL-Maackiain (Maackiain) is extracted from the flowers of maackia amurensis rupr.et maxim. and can induce apoptosis and growth suppression. Maackiain (DL-Maackiain) is a larvicidal agent against Aedes aegypti mosquito.xp Parasitol with a LD50 of  21.95 µg/mL[3]. Extent of induction of apoptosis by maackiain was larger than that by trifolirhizin in human leukemia HL-60 cells[4]. MK (Maackiain) significantly reduced dopaminergic neuron damage in 6-hydroxydopamine (6-OHDA)-exposed worms of the BZ555 strain, with corresponding improvements in food-sensing behavior and life-span. In transgenic worms of strain NL5901 treated with 0.25 mM MK, the accumulation of α-synuclein was diminished by 27% compared with that in untreated worms. Moreover, in worms and the SH-SY5Y cell line, we confirmed that the mechanism of MK-mediated protection against PD pathology may include blocking apoptosis, enhancing the ubiquitin-proteasome system, and augmenting autophagy by increasing PINK1/parkin expression[5]. Maackiain could restrain receptor activator of nuclear factor-κB ligand (RANKL)-stimulated osteoclast formation and hydroxyapatite resorption dose-dependently, and interrupt the structures of F-actin belts in the mature osteoclasts. It also repressed the expressions of osteoclast-specific genes and proteins. Furthermore, Maackiain could inhibit RANKL-stimulated NF-κB and calcium signalling pathways, and dampen Nuclear factor of activated T cell cytoplasmic 1 activity, protein expression and translocation into the nucleus[6].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.52mL

0.70mL

0.35mL

17.59mL

3.52mL

1.76mL

35.18mL

7.04mL

3.52mL
参考文献

[1]Bezerra-Silva PC, Santos JC, et al. Extract of Bowdichia virgilioides and maackiain as larvicidal agent against Aedes aegypti mosquito. Exp Parasitol. 2015 Jun;153:160-4.

[2]Gao S, Yang Z, et al. Validated LC-MS/MS method for the determination of maackiain and its sulfate and glucuronide in blood: application to pharmacokinetic and disposition studies. J Pharm Biomed Anal. 2011 May 15;55(2):288-93.

[3]Bezerra-Silva PC, Santos JC, Santos GK, Dutra KA, Santana AL, Maranhão CA, Nascimento MS, Navarro DM, Bieber LW. Extract of Bowdichia virgilioides and maackiain as larvicidal agent against Aedes aegypti mosquito. Exp Parasitol. 2015 Jun;153:160-4

[4]Aratanechemuge Y, Hibasami H, Katsuzaki H, Imai K, Komiya T. Induction of apoptosis by maackiain and trifolirhizin (maackiain glycoside) isolated from sanzukon (Sophora Subprostrate Chen et T. Chen) in human promyelotic leukemia HL-60 cells. Oncol Rep. 2004 Dec;12(6):1183-8

[5]Tsai RT, Tsai CW, Liu SP, Gao JX, Kuo YH, Chao PM, Hung HS, Shyu WC, Lin SZ, Fu RH. Maackiain Ameliorates 6-Hydroxydopamine and SNCA Pathologies by Modulating the PINK1/Parkin Pathway in Models of Parkinson's Disease in Caenorhabditis elegans and the SH-SY5Y Cell Line. Int J Mol Sci. 2020 Jun 23;21(12):4455

[6]Liu Y, Zeng W, Ma C, Wang Z, Wang C, Li S, He W, Zhang Q, Xu J, Zhou C. Maackiain dampens osteoclastogenesis via attenuating RANKL-stimulated NF-κB signalling pathway and NFATc1 activity. J Cell Mol Med. 2020 Nov;24(21):12308-12317