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Aftin-4

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Chemical Structure| 866893-90-5 同义名 : -
CAS号 : 866893-90-5
货号 : A107870
分子式 : C20H28N6O
纯度 : 98%+
分子量 : 368.476
MDL号 : MFCD30187584
存储条件：

粉末 Keep in dark place,Inert atmosphere,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(284.96 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：

IP 2% DMSO+2% Tween80+30% PEG300+water 10 mg/mL clear

PO 0.5% CMC-Na 63 mg/mL suspension

生物活性
描述 Aftin-4 is an Aβ1−42 inducer which could result in a rapid, acute Alzheimer’s disease-like toxicity in the rodent brain in vivo. It promoted the generation of amyloid-β (Aβ)1−42 from amyloid-β protein precursor through γ-secretase activation in cell cultures. The intracerebroventricular administration of aftin-4 at 3–20nM increased Aβ1−42, but not Aβ1-40, content in the mouse hippocampus, between 5 and 14 days after injection. The treatment provoked marked learning deficits. Also it increased brain contents in pro-inflammatory cytokines, IL-1β, IL-6, and TNFα, and GFAP immunolabeling, showing astrocytic reaction, as well as increased lipid peroxidation levels in the hippocampus, an index of oxidative stress. Expression of the synaptic marker synaptophysin was decreased by aftin-4. The systemic intraperitoneal injection of aftin-4 in the 3-30 mg/kg dose range also induced oxidative stress and learning deficits[2].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.71mL

0.54mL

0.27mL

13.57mL

2.71mL

1.36mL

27.14mL

5.43mL

2.71mL
参考文献

[1]Meunier J, Borjini N, et al. Brain toxicity and inflammation induced in vivo in mice by the amyloid-β forty-two inducer aftin-4, a roscovitine derivative. J Alzheimers Dis. 2015;44(2):507-24.

[2]Meunier J, Borjini N, Gillis C, Villard V, Maurice T. Brain toxicity and inflammation induced in vivo in mice by the amyloid-β forty-two inducer aftin-4, a roscovitine derivative. J Alzheimers Dis. 2015;44(2):507-24. doi: 10.3233/JAD-140711. PMID: 25298201.