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Genistein

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Chemical Structure| 446-72-0 同义名 : 金雀异黄酮 ;NPI 031L;CI-75610;BIO300;SIPI-9764-I;PTI-G-4660;G-2535;Genistein, BIO-300;NSC 36586
CAS号 : 446-72-0
货号 : A107459
分子式 : C15H10O5
纯度 : 98%
分子量 : 270.237
MDL号 : MFCD00016952
存储条件：

粉末 Sealed in dry,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(388.55 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
靶点

  • EGFR/ErbB1

  • Topo II
描述 Genistein is an isoflavone present in soy and is known to have multiple molecular effects, such as the inhibition of inflammation, promotion of apoptosis, and modulation of steroidal hormone receptors and metabolic pathways[3]. Genistein is the natural isoflavone and a phytoestrogen with a broad range of pharmacological properties, such as tyrosine and topoisomerase inhibition. It also induces apoptosis and cell proliferation inhibition, differentiates cancer cells. Added health benefits include the reduction of osteoporosis by suppressing osteoclasts and lymphocyte functions, decreased the risk of cardiovascular attacks and relieved postmenopausal problems[4]. Retardation of atherogenic activity and increasing the antioxidant defense of a cell has been attributed to genistein while as it has also been reported that genistein possesses suppressive effects on both the cell-mediated and humoral components of the adaptive immune system. In addition, genistein has its prominent role in preventing the DNA damage. Genistein confers a better protection to ischemic conditions thereby giving a significant cardioprotection[5]. Gensitein possesses cancer related enzyme-inhibitory effect and substantially inhibits skin carcinogenesis and cutaneous aging induced by ultraviolet (UV) light in mice and photodamage in humans. The anticancer, anti-inflammatory, cardio-protective and enzyme-inhibitory effects of genistein might be related to their antioxidant activities[6].
细胞研究
细胞系 浓度 检测类型 检测时间 活动说明 数据源
293T cells Function assay Displacement of [3H]17beta-estradiol from recombinant human ERbeta expressed in 293T cells, IC50=0.0013 μM 16219463
3T3 cells Cytotoxic assay Cytotoxic effect on 3T3 cells, IC50=24 μM 8201603
A431 cells 50 to 75 uM Function assay 36 h Cell cycle arrest in human A431 cells assessed as decrease in accumulation at G0/G1 phase at 50 to 75 uM after 36 hrs by propidium iodide staining-based flow cytometric analysis 24456004
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.70mL

0.74mL

0.37mL

18.50mL

3.70mL

1.85mL

37.00mL

7.40mL

3.70mL
参考文献

[1]Jalili C, Ahmadi S, et al. Effect of Genistein on reproductive parameter and serum nitric oxide levels in morphine-treated mice. Int J Reprod Biomed (Yazd). 2016 Feb;14(2):95-102.

[2]Akiyama T, Ishida J, et al. Genistein, a specific inhibitor of tyrosine-specific protein kinases. J Biol Chem. 1987 Apr 25;262(12):5592-5.

[3]Mukund V, Mukund D, Sharma V, Mannarapu M, Alam A. Genistein: Its role in metabolic diseases and cancer. Crit Rev Oncol Hematol. 2017 Nov;119:13-22

[4]Jaiswal N, Akhtar J, Singh SP; Badruddeen, Ahsan F. An Overview on Genistein and its Various Formulations. Drug Res (Stuttg). 2019 Jun;69(6):305-313

[5]Ganai AA, Farooqi H. Bioactivity of genistein: A review of in vitro and in vivo studies. Biomed Pharmacother. 2015 Dec;76:30-8

[6]Rahman Mazumder MA, Hongsprabhas P. Genistein as antioxidant and antibrowning agents in in vivo and in vitro: A review. Biomed Pharmacother. 2016 Aug;82:379-92