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Insulin(cattle)

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Chemical Structure| 11070-73-8 同义名 : 胰岛素, 来源于牛胰腺;胰岛素(牛) ;Insulin from bovine pancreas
CAS号 : 11070-73-8
货号 : A1002085
分子式 : C254H377N65O75S6
纯度 : 98%
分子量 : 5733.49
MDL号 : MFCD02321930
存储条件：

粉末 Inert atmosphere,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

H2O: 40 mg/mL(6.98 mM)，配合低频超声，并调节pH至2
动物实验配方：
生物活性
描述 Insulin, Bovine is a two-chain polypeptide hormone produced in vivo in the pancreatic β cells. Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates glucose uptake into muscle and fat cells by recruiting membrane glucose transporter Glut-4 to cell surface. Insulin cattle has often been used as growth supplement in culturing cells at the concentration ranging from 1 to 10 μg/mL of medium[1]. A stability experiment on purified crystalline bovine insulin showed that it deteriorates at normal refrigeration temperature (5 degrees C) and that storage at -20 degrees C is necessary to ensure stability. Purified insulin degrades by two mechanisms: deamidation and polymerization. Immunochemical potency determinations alone are insufficient to determine the stability of insulin as they may be little affected by the chemical changes which occur on storage. The activation energy for the polymerization of insulin was found to be 66 kJ/mol and for deamidation 45 kJ/mol. It is recommended that crystalline bovine insulin be stored at -20 degrees C[2].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

0.17mL

0.03mL

0.02mL

0.87mL

0.17mL

0.09mL

1.74mL

0.35mL

0.17mL
参考文献

[1]Yousefi R, Taheri B, Alavi P, et al. Aspirin-mediated acetylation induces structural alteration and aggregation of bovine pancreatic insulin. J Biomol Struct Dyn. 2016;34(2):362-375

[2]Fisher BV, Porter PB. Stability of bovine insulin. J Pharm Pharmacol. 1981;33(4):203-206